N-[[1-(5-fluoropentyl)-1H-indazol-3-yl]carbonyl]-three-methyl-D-valine methyl ester (5F-ADB) is one of the maximum strong synthetic cannabinoids and elicits extreme psychotic symptoms in human beings, occasionally inflicting death.
to research the neuronal mechanisms underlying its toxicity, we tested the consequences of 5F-ADB on midbrain dopaminergic and serotonergic structures, which modulate numerous primary mind features including the ones in praise–associated behavior. 5F-ADB-induced modifications in spontaneous firing activity of dopaminergic and serotonergic neurons were recorded via ex vivo electrophysiological strategies.
In dopaminergic neurons, 5F-ADB (1 μM) considerably expanded the spontaneous firing charge, whilst 5F-ADB didn’t spark off dopaminergic neurons inside the presence of the CB1 antagonist AM251 (1 μM). however, the identical concentration of 5F-ADB did now not affect serotonergic-neuron hobby. those outcomes advise that 5F-ADB activates nearby CB1 receptors and potentiates midbrain dopaminergic structures with no direct results on midbrain serotonergic structures.